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. 2017 Jun 28;10:205. doi: 10.3389/fnmol.2017.00205

Figure 1.

Figure 1

Expression of a subset of itch-related molecules in Tlx3cko dorsal root ganglions (DRGs) and spinal cords. In situ hybridizations with indicated probes were performed on transverse sections of lumbar DRGs (A,B) and spinal cords (C) from adult control and Tlx3cko mice, the statistical data were shown on (D,E). Compared with that in control mice, expression of MrgprA3, IL31ra, Nppb and Sst were almost completely eliminated in Tlx3cko mice (A). The percentage of MrgprD+ neurons in Tlx3cko mice was markedly reduced (control, 32.2% ± 1.7%, n = 3; Tlx3cko, 13.8% ± 0.4%, n = 3; p = 0.0004). The percentage of MrgprC11+ neurons was greatly increased in Tlx3cko mice (control, 8% ± 0.1%, n = 3; Tlx3cko, 28.5% ± 1.3%, n = 3; p < 0.0001) (B). (C) The numbers of GRP+ and GRPR+ neurons in the spinal cords of Tlx3cko and control mice were comparable (GRP+ neruons : control, 32 ± 1/section; Tlx3cko 34 ± 1/section; n = 28–32 sections from 3 mice, p = 0.3753; GRPR+ neurons: control, 14 ± 1/section; Tlx3cko 13 ± 1/section; n = 22–23 sections from 3 mice, p = 0.8294). ***Indicates p < 0.001, Scale bar represents 100 μm.