Table 2.

Sequences and annealing temperatures for molecular markers associated with resistance genes used in the study.

Marker name (gene)	Primer sequence (5′–3′)	Annealing temperature (°C)	Reference
Xcfd19 (Lr32)	TACGCAGGTTTGCTGCTT CT	59	Guyomarc’h et al., 2002
	GGAGTTCACAAGCATGGGTT
Xksu-D14 (Lr32)	CGCTTTTACCGAGATTGGTC	59	Boyko et al., 1999
	CCAAAGAGCATCCATGGTGT
Xgwm296 (Lr22a, Lr39)	AATTCAACCTACCAATCTCTG	52	Roder et al., 1998
	GCCTAATAAACTGAAAACGAG
Xgdm35 (Lr22a, Lr39)	CCTGCTCTGCCCTAGATACG	60	Pestsova et al., 2000
	ATGTGAATGTGATGCATGCA
Xcfd4 (Lr32)	TGCTCCGTCTCCGAGTAGAT	59	Guyomarc’h et al., 2002
	GGGAAGGAGAGATGGGAAAC
Xbarc135 (Lr32)	ATCGCCATCTCCTCTACCA	58	Song et al., 2005
	GCGAACCCATGTGCTAAGT
Xgwm539 (Pm43 homolog)	CTGCTCTAAGATTCATGCAACC	58	Roder et al., 1998
	GAGGCTTGTGCCCTCTGTAG