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. 2017 Jun 28;8:1193. doi: 10.3389/fmicb.2017.01193

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Copyright © 2017 Dewachter, Verstraeten, Jennes, Verbeelen, Biboy, Monteyne, Pérez-Morga, Verstrepen, Vollmer, Fauvart and Michiels.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Amidases and the Tol-Pal system do not directly cause ObgE^∗-mediated lysis. (A) Induced cultures of E. coli (wt), E. coli ΔamiA ΔamiB ΔamiC (ΔamiABC), E. coli ΔtolQ and E. coliΔtolR with plasmids pBAD33, pBAD33-obgE or pBAD33-obgE^∗ were stained with PI and the percentage of PI-negative and thus intact cells in the population was measured by flow cytometry. (B) The percentage of intact cells upon ObgE^∗ expression was divided by the fraction of intact cells upon expression of ObgE to correct for the differences in baseline levels of membrane integrity in different strains. Data are represented as mean ± SEM, n ≥ 3. In every repeat 100,000 cells were collected (one-way ANOVA, Bonferroni correction: ^∗p < 0.05, ^∗∗∗∗p < 0.0001).