FIGURE 5.

Arrangement of the mtrAB-lpqB operon in S. venezuelae NRRL B-65442 and Streptomyces coelicolor M145. Published dRNA-seq data (Jeong et al., 2016; Munnoch et al., 2016) shows that the major transcriptional start site is at +1 in both species, relative to the mtrA translational start codon. A second transcript starts at –79 in S. venezuelae and an MtrA ChIP peak is centered between P1 and P2 at –50, suggesting autoregulation. In S. coelicolor a gene encoding a putative eukaryotic translation initiation factor has inserted immediately upstream of mtrA and is also expressed on a leaderless transcript.