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Neurotransmitter of JO neurons. JO neurons labeled with anti-ChAT (A), anti-serotonin (5-HT) (B), anti-GABA (C), and anti-glutamate (Glu) (D) antibodies (green signals). The nuclei of all JO neurons were labeled with anti-ELAV antibody (magenta signals). vGluT positive neurons were labeled by a combination of vGluT-GAL4 driver and UAS-RedStinger marker strains (blue signals) (D). D, dorsal; M, medial.
