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. 2017 Jun 28;23(24):4341–4353. doi: 10.3748/wjg.v23.i24.4341

Table 1.

Flow cytometry staining

Analysis Staining solution Manufacturer
Caspase activation Caspase-3-PE BD Biosciences Pharmingen, San Diego, CA, United States
Cleaved Caspase-9-FITC BIOSS Antibodies, Woburn, MA, United States
Cytochrome c release Cytochrome c Antibody (6H2) FITC Conjugate Invitrogen, Carlsbad, CA, United States
Cleavage of PARP Cleaved-PARP (Asp214) XP® Rabbit mAb (PE Conjugate) Cell Signaling Technology, Danvers, MA, United States
Mitochondrial membrane potential TMRE (tetramethylrhodamine ethyl ester perchlorate) Final concentration 0.1 µmol/L Sigma-Aldrich, St. Louis, Missouri, United States
Protein analysis Phospho-Bcl-2 (Ser70) Rabbit mAb Alexa Fluor® 488 Conjugate Cell Signaling Technology®, Danvers, MA, United States
Phospho-Bad (Ser112) Rabbit mAb (PE Conjugate)