Caspase activation |
Caspase-3-PE |
BD Biosciences Pharmingen, San Diego, CA, United States |
Cleaved Caspase-9-FITC |
BIOSS Antibodies, Woburn, MA, United States |
Cytochrome c release |
Cytochrome c Antibody (6H2) FITC Conjugate |
Invitrogen, Carlsbad, CA, United States |
Cleavage of PARP |
Cleaved-PARP (Asp214) XP® Rabbit mAb (PE Conjugate) |
Cell Signaling Technology, Danvers, MA, United States |
Mitochondrial membrane potential |
TMRE (tetramethylrhodamine ethyl ester perchlorate) Final concentration 0.1 µmol/L |
Sigma-Aldrich, St. Louis, Missouri, United States |
Protein analysis |
Phospho-Bcl-2 (Ser70) Rabbit mAb Alexa Fluor® 488 Conjugate |
Cell Signaling Technology®, Danvers, MA, United States |
Phospho-Bad (Ser112) Rabbit mAb (PE Conjugate) |