Table 1.
Details of primers used for real-time PCR analysis
| Target | Sequence | Annealing, °C | Oligo concentration, nmol/L | Fragment size, bp | NCBI reference sequence | cDNA concentration, μL |
| GAPDH | F TGACTCTACCCACGGCAAGTTCAA | 60 | 200 | 141 | NM_017008.3 | 1.000 |
| R ACGACATACTCAGCACCAGCATCA | ||||||
| β-actin | F TTGCTGACAGGATGCAGAAGGAGA | 60 | 100 | 159 | NM_031144.2 | 1.000 |
| R ACTCCTGCTTGCTGATCCACATCT | ||||||
| HPRT | F AGGGAAGTGACAATCTACCTGACG | 60 | 100 | 81 | AA900579.1 | 0.125 |
| R GAAATGTCTGTTGCTGCGTCCCTT | ||||||
| HSP70 | F ACTCCTTCGTTCGGTCTGCAATCA | 60 | 200 | 92 | NM_031971.2 | 0.125 |
| R CTGGGAATGCAAAGCACACGTGAA | ||||||
| Heparanase | F TGTCAAGAGTGAAAGGCCCAGACA | 60 | 200 | 141 | NM_022605.1 | 0.125 |
| R GCAGCTTCAAGTGCTTGGTGACAT | ||||||
| NF-κB | F AAACCAAAGCCCTGAAAGGCCATC | 60 | 200 | 120 | XM_342346.4 | 0.125 |
| R TCGGAAGGCCTCGAATGACATCAA | ||||||
| MAPK1 | F AACAGGTTGTTCCCAAACGCTGAC | 60 | 200 | 187 | NM_053842.1 | 0.500 |
| R AGTCGTCCAGCTCCATGTCAAACT | ||||||
| MAPK3 | F TACCTGGACCAGCTCAACCACATT | 60 | 200 | 173 | NM_017347.2 | 0.500 |
| R AGCAGGTCAAGAGCTTTGGAGTCA | ||||||
| MAPK6 | F AACTGAGCCAGTGGAAGAAGGGAA | 60 | 200 | 164 | NM_031622.2 | 1.000 |
| R TTAACGTGGCCTGGATGGACTTGA | ||||||
| MAPK9 | F TCATGGGAGAGCTGGTGAAAGGTT | 60 | 200 | 106 | NM_017322.2 | 1.000 |
| R ATGAACTCTGCGGATGGTGTTCCT | NM_001270544.1 | |||||
| NM_001270545.1 | ||||||
| MUC1 | F CCGCTACTACCAAGAACTGAAG | 60 | 200 | 102 | NM_012602.1 | 0.500 |
| R GAGCCTGACCTGAACTTGATAG | ||||||
| MUC2 | F GGCTCTGCTCTCTGTGTTATAG | 60 | 200 | 123 | U68172.1 | 1.000 |
| R CAGTTTGGGAAGAAGGTAGGG | ||||||
| MUC3 | F GGGAAATAGACCCTGCAGTTAG | 60 | 200 | 107 | U76551.1 | 0.125 |
| R GATCATCGCTTGCCGTCATA | ||||||
| MUC4 | F ATGTGGAGGTGGGAGAAATG | 60 | 200 | 122 | AF240632.1 | 0.500 |
| R CCCTGGAACTGGAATTAGAGAC |