Table 2.
Fermentation parameters after 24 h of incubation of a mixed diet in a foregut model of rumen fermentation incubated with kefir (CTR), unaltered kefir (KEF), autoclaved kefir (AUT), or pasteurized kefir (PAS).
| CTR | KEF | AUT | PAS | SED | Significance | |
|---|---|---|---|---|---|---|
| Fermentation products | ||||||
| Total VFA (mM) | 60.0c | 63.2ab | 61.8b | 64.4a | 0.793 | ∗∗ |
| Acetate (%) | 62.1a | 60.2c | 60.5b | 60.2c | 0.096 | ∗∗∗ |
| Propionate (%) | 21.3b | 21.5a | 21.6a | 21.6a | 0.058 | ∗∗ |
| N-Butyrate (%) | 1.02b | 1.16a | 1.15a | 1.14a | 0.023 | ∗∗∗ |
| C2:C3 ratio | 2.91a | 2.80b | 2.81b | 2.79b | 0.010 | ∗∗∗ |
| NH3-N (mM) | 8.36b | 10.23a | 10.29a | 10.14a | 0.364 | ∗∗∗ |
| Lactate (mM) | 0.38c | 0.63bc | 0.86ab | 0.94a | 0.150 | ∗ |
| Microbiology (log CFU +1) | ||||||
| LAB† | 5.74 | 5.38 | 5.37 | 5.52 | 0.176 | NS |
| AAB† | 3.24 | 3.52 | 3.37 | 3.67 | 0.180 | NS |
| Yeast | 2.89 | 2.94 | 2.94 | 2.93 | 0.061 | NS |
| Fermentation parameters | ||||||
| pH | 6.61a | 6.52b | 6.48b | 6.48b | 0.039 | ∗ |
| c¥ | 0.05 | 0.06 | 0.06 | 0.06 | 0.003 | NS |
| a+b∓ | 96.2b | 106.6a | 109.1a | 108.2a | 3.691 | ∗ |
SED means Standard error of the difference between means (N = 4). ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001; NS means P > 0.1. †LAB, lactic acid bacteria; AAB, acid acetic bacteria. ¥Gas production rate, according to the following formula: Y = a + b (1 - e(–ct)). ∓Maximum potential of fermentation, expressed in mL of gas per gr of substrate. Within a row, means without a common superscript differ (P < 0.05).