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. 2017 Jun 28;8:1194. doi: 10.3389/fmicb.2017.01194

Table 2.

Fermentation parameters after 24 h of incubation of a mixed diet in a foregut model of rumen fermentation incubated with kefir (CTR), unaltered kefir (KEF), autoclaved kefir (AUT), or pasteurized kefir (PAS).

CTR KEF AUT PAS SED Significance
Fermentation products
Total VFA (mM) 60.0c 63.2ab 61.8b 64.4a 0.793 ∗∗
Acetate (%) 62.1a 60.2c 60.5b 60.2c 0.096 ∗∗∗
Propionate (%) 21.3b 21.5a 21.6a 21.6a 0.058 ∗∗
N-Butyrate (%) 1.02b 1.16a 1.15a 1.14a 0.023 ∗∗∗
C2:C3 ratio 2.91a 2.80b 2.81b 2.79b 0.010 ∗∗∗
NH3-N (mM) 8.36b 10.23a 10.29a 10.14a 0.364 ∗∗∗
Lactate (mM) 0.38c 0.63bc 0.86ab 0.94a 0.150
Microbiology (log CFU +1)
LAB 5.74 5.38 5.37 5.52 0.176 NS
AAB 3.24 3.52 3.37 3.67 0.180 NS
Yeast 2.89 2.94 2.94 2.93 0.061 NS
Fermentation parameters
pH 6.61a 6.52b 6.48b 6.48b 0.039
c¥ 0.05 0.06 0.06 0.06 0.003 NS
a+b 96.2b 106.6a 109.1a 108.2a 3.691

SED means Standard error of the difference between means (N = 4). P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001; NS means P > 0.1. †LAB, lactic acid bacteria; AAB, acid acetic bacteria. ¥Gas production rate, according to the following formula: Y = a + b (1 - e(–ct)). Maximum potential of fermentation, expressed in mL of gas per gr of substrate. Within a row, means without a common superscript differ (P < 0.05).