Table 3.
Cell counts | Incubation times (h) | SEM | Effects | ||||
---|---|---|---|---|---|---|---|
24 | 48 | 72 | A | Time | A*T | ||
Control | 12080a | 19395a | 19999a | 1440 | |||
AMPICILLIN | |||||||
0.1 | 0.83 | 0.47ab | 0.52ab | 0.12 | <0.0001 | <0.0001 | 0.0066 |
0.5 | 0.84 | 0.01b | 0.01b | 0.14 | |||
1 | 0.83 | 0.43ab | 0b | 0.13 | |||
2 | 0.85 | 0.63a | 0.47ab | 0.06 | |||
Contrast | – | Q*** | L*,Q*** | ||||
STREPTOMYCIN | |||||||
0.1 | 0.82 | 1.03a | 1.11a | 0.07 | <0.0001 | 0.257 | 0.0031 |
0.5 | 0.95 | 0.89a | 0.98ab | 0.03 | |||
1 | 0.69 | 0.55b | 0.76b | 0.05 | |||
2 | 0.88 | 0.49b | 0.42c | 0.08 | |||
Contrast | – | L***,Q* | L*** | ||||
CARBENICILLIN | |||||||
0.1 | 0.83 | 0.64b | 0.85a | 0.04 | <0.0001 | <0.0001 | 0.0004 |
0.5 | 0.87 | 0.45b | 0.43b | 0.07 | |||
1 | 0.93 | 0.56b | 0.44b | 0.08 | |||
2 | 0.78 | 0.49b | 0.28b | 0.08 | |||
Contrast | - | L***,Q*** | L***,Q*** | ||||
TETRACYCLINE | |||||||
0.03 | 0.94 | 0.58b | 0.2b | 0.11 | <0.0001 | <0.0001 | 0.0001 |
0.05 | 0.87 | 0.49bc | 0.23b | 0.1 | |||
0.1 | 0.88 | 0.55b | 0.17b | 0.11 | |||
0.3 | 0.81 | 0.24c | 0.03b | 0.12 | |||
Contrast | - | L***,Q** | L***,Q*** | ||||
NEOMYCIN | |||||||
0.1 | 0.85 | 0.75b | 0.96a | 0.04 | <0.0001 | <0.0001 | 0.0011 |
0.3 | 0.84 | 0.53bc | 0.51b | 0.06 | |||
0.6 | 0.76 | 0.51c | 0.37b | 0.06 | |||
1 | 0.74 | 0.42c | 0.33b | 0.07 | |||
Contrast | L* | L***,Q*** | L***,Q*** | ||||
BACITRACIN | |||||||
0.05 | 0.89ab | 0.72ab | 0.56b | 0.07 | <0.0001 | <0.0001 | 0.0514 |
0.1 | 0.93ab | 0.58b | 0.5b | 0.08 | |||
0.5 | 0.71ab | 0.54b | 0.4b | 0.05 | |||
1 | 0.49b | 0.07c | 0.01c | 0.08 | |||
Contrast | L*** | L*** | L*** | ||||
NORMOCIN™ | |||||||
0.05 | 0.89ab | 0.65b | 0.56b | 0.06 | <0.0001 | <0.0001 | 0.2012 |
0.1 | 0.69ab | 0.47bc | 0.57b | 0.04 | |||
0.3 | 0.69ab | 0.39bc | 0.31c | 0.07 | |||
0.5 | 0.5b | 0.18c | 0.1c | 0.08 | |||
Contrast | L*** | L***,Q** | L***,Q** | ||||
CHLORAMPHENICOL | |||||||
0.005 | 0.82a | 0.11b | 0.08b | 0.13 | <0.0001 | <0.0001 | <0.0001 |
0.01 | 0.76ab | 0b | 0b | 0.13 | |||
0.03 | 0.71ab | 0b | 0b | 0.12 | |||
0.05 | 0.39b | 0b | 0b | 0.07 | |||
Contrast | L*** | L***,Q*** | L***,Q*** |
The final concentrations of antibiotics were in mg/ml. Different superscripts (a–c) in the same column denote significant difference (P < 0.05) in the proportion of protozoa cell counts between the control and each antibiotic treatment. Significance (
, P ≤ 0.01;
, P ≤ 0.05;
, P ≤ 0.10) of linear (L) and quadratic (Q) contrast are shown. Each value was the mean of three replicates. A, antibiotics; T, incubation time.