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. 2017 Jun 28;8:1189. doi: 10.3389/fmicb.2017.01189

Table 3.

E. caudatum data (% of that of the control) in the presence of different antibiotics (mg/ml).

Cell counts Incubation times (h) SEM Effects
24 48 72 A Time A*T
Control 12080a 19395a 19999a 1440
AMPICILLIN
0.1 0.83 0.47ab 0.52ab 0.12 <0.0001 <0.0001 0.0066
0.5 0.84 0.01b 0.01b 0.14
1 0.83 0.43ab 0b 0.13
2 0.85 0.63a 0.47ab 0.06
Contrast Q*** L*,Q***
STREPTOMYCIN
0.1 0.82 1.03a 1.11a 0.07 <0.0001 0.257 0.0031
0.5 0.95 0.89a 0.98ab 0.03
1 0.69 0.55b 0.76b 0.05
2 0.88 0.49b 0.42c 0.08
Contrast L***,Q* L***
CARBENICILLIN
0.1 0.83 0.64b 0.85a 0.04 <0.0001 <0.0001 0.0004
0.5 0.87 0.45b 0.43b 0.07
1 0.93 0.56b 0.44b 0.08
2 0.78 0.49b 0.28b 0.08
Contrast - L***,Q*** L***,Q***
TETRACYCLINE
0.03 0.94 0.58b 0.2b 0.11 <0.0001 <0.0001 0.0001
0.05 0.87 0.49bc 0.23b 0.1
0.1 0.88 0.55b 0.17b 0.11
0.3 0.81 0.24c 0.03b 0.12
Contrast - L***,Q** L***,Q***
NEOMYCIN
0.1 0.85 0.75b 0.96a 0.04 <0.0001 <0.0001 0.0011
0.3 0.84 0.53bc 0.51b 0.06
0.6 0.76 0.51c 0.37b 0.06
1 0.74 0.42c 0.33b 0.07
Contrast L* L***,Q*** L***,Q***
BACITRACIN
0.05 0.89ab 0.72ab 0.56b 0.07 <0.0001 <0.0001 0.0514
0.1 0.93ab 0.58b 0.5b 0.08
0.5 0.71ab 0.54b 0.4b 0.05
1 0.49b 0.07c 0.01c 0.08
Contrast L*** L*** L***
NORMOCIN
0.05 0.89ab 0.65b 0.56b 0.06 <0.0001 <0.0001 0.2012
0.1 0.69ab 0.47bc 0.57b 0.04
0.3 0.69ab 0.39bc 0.31c 0.07
0.5 0.5b 0.18c 0.1c 0.08
Contrast L*** L***,Q** L***,Q**
CHLORAMPHENICOL
0.005 0.82a 0.11b 0.08b 0.13 <0.0001 <0.0001 <0.0001
0.01 0.76ab 0b 0b 0.13
0.03 0.71ab 0b 0b 0.12
0.05 0.39b 0b 0b 0.07
Contrast L*** L***,Q*** L***,Q***

The final concentrations of antibiotics were in mg/ml. Different superscripts (a–c) in the same column denote significant difference (P < 0.05) in the proportion of protozoa cell counts between the control and each antibiotic treatment. Significance (

***

, P ≤ 0.01;

**

, P ≤ 0.05;

*

, P ≤ 0.10) of linear (L) and quadratic (Q) contrast are shown. Each value was the mean of three replicates. A, antibiotics; T, incubation time.