FIG 1.

Characterization of clones with integrated HHV-6. (A and B) DNA samples from U2OS and a U2OS-BP6 clone containing ciHHV-6B were analyzed by ddPCR. After PCR, the content of droplets was analyzed for the presence of HHV-6 (blue), cellular gene RPP30 (green), HHV-6⁺ RPP30 (orange), or no DNA (gray). (C) The average number of HHV-6 copies/cell from U2OS clones with ciHHV-6A and ciHHV-6B was determined by ddPCR. DNA from cell clones without HHV-6 integration and DNA samples from known iciHHV-6A⁺/B⁺ subjects were used as negative and positive controls (neg and pos ctl), respectively. (D) FISH analysis of a U2OS clone containing ciHHV-6A. Telomeres (TMR) and HHV-6A were visualized using Cy3-labeled telomeric probe (red) and HHV-6A using Alexa-488 (green)-labeled probe. An enlarged portion of the image is presented on the right.