FIG 3.

Spontaneous HHV-6 gene expression from ciHHV-6A/B U2OS clones. (A) Eleven U2OS clones containing ciHHV-6A were analyzed for the spontaneous expression of IE and U100 by RT-qPCR. Results are expressed as mean ratios ± SD of viral genes relative to GAPDH mRNA levels. (B) U2OS-AP3 (ciHHV-6A⁺) and U2OS-BP6 (ciHHV-6B⁺) were analyzed for the spontaneous expression of IE1 protein by IFA using purified anti-IE1 rabbit IgG coupled to Alexa 488. Nuclei were stained blue using 4′,6-diamidino-2-phenylindole (DAPI).