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. 2017 Jun 26;91(14):e00437-17. doi: 10.1128/JVI.00437-17

FIG 4.

FIG 4

Spontaneous and induced HHV-6 gene expression from ciHHV-6A+/B+ U2OS and MCF-7 clones. (A to F) The expression of HHV-6A/B U90, U39, and U100 genes from ciHHV-6A+ (AP3 and WTR-BAC#25) and ciHHV-6B+ (BP6) U2OS clones was determined by RT-qPCR at the indicated times following TNT (TPA, NaBy, TSA, and hydrocortisone) or SAHA stimulation. Results are shown as mean fold induction ± SD relative to untreated (resting) cells. (G to I) Expression of U90, U39, and U100 from ciHHV-6A+ (MCF7-66A) and ciHHV-6B+ (MCF-7 32B) MCF-7 clones was determined by RT-qPCR at the indicated times following TNT or SAHA stimulation. Results are shown as mean fold induction ± SD relative to untreated (resting) cells. NS, not stimulated. (J) GFP expression in resting and TSA-activated U2OS clone 9 containing integrated WTR-BAC HHV-6A. Results are representative of a minimum of three independent experiments performed in triplicate. *, P < 0.05; **, P < 0.01.