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HSV-1 sustains mTORC1 activity in cells exposed to energy insufficiency. NHDFs growth arrested by serum deprivation were mock infected or infected with wild-type (WT) HSV-1 (strain F). Cultures were either untreated (−) or treated (+) with AICAR at 12 hpi. Total protein was isolated at 15 hpi, fractionated by SDS-PAGE, and analyzed by immunoblotting using the indicated antibodies. M, mock.
