FIG 1.

Three antibodies specific to NS5A phosphorylation at S222, S235, and S238 were characterized. (A) Partial peptide sequences of the LCS I region of NS5A from six major HCV genotypes. The UniProt accession numbers are the following: 1a, P27958; 2a, Q99IB8; 3a, Q81258; 4a, O39929; 5a, O91936; and 6a, O39927. Phosphorylation events at S222, S235, and S238 were identified with high confidence scores in a previous study (22). (B) Dot blot tests for the specificity of the three antibodies. Synthetic peptides with or without phosphorylation at the designated serine residues (numbered) were dotted on nitrocellulose membranes before detection with the antibodies.