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. 2017 Jun 26;91(14):e00194-17. doi: 10.1128/JVI.00194-17

FIG 4.

FIG 4

NS5A S235 phosphorylation could occur alone, while S238 phosphorylation occurred only with S235 phosphorylation on the same NS5A molecule. (A) Immunoblots for NS5A and NS5A phosphorylation at S235 (left) or S238 (right) in the immunoprecipitate (IP) of the S235 phosphorylation-specific antibody. (B) Immunoblots for NS5A and NS5A phosphorylation at S235 (left) or S238 (right) in the immunoprecipitate of the S238 phosphorylation-specific antibody. In, input, i.e., HCV (J6/JFH1)-infected Huh7.5.1 cell lysate; Unb, unbound; W, wash; E, eluate.