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. 2017 Jun 26;91(14):e00194-17. doi: 10.1128/JVI.00194-17

FIG 6.

FIG 6

NS5A S235 phosphorylation is essential for HCV replication. Confocal immunofluorescence micrographs of NS5A phosphorylated at S235 or S238 costained for dsRNA (A) or lipid droplet (B) in the wild-type (WT) JFH1 replicon-transfected Huh7.5.1 cells. (C and D) Confocal immunofluorescence micrographs of NS5A phosphorylated at S235 or S238 costained for dsRNA in the Huh7.5.1 cells transfected with S235A (C) or S238A (D) mutant replicon. Nuclei were stained with DAPI (in blue). Boxes indicates the areas enlarged.