FIG 8.

CKIα inhibition reduced NS5A phosphorylation at S235 and S238. Immunoblots for NS5A phosphorylated at S235 (A) and S238 (B) in T7-Huh7 cells. The cells were transfected with an NS3-NS5B expression construct and treated with the CKIα inhibitor D4476 for 1 day prior to immunoblotting. β-Actin served as a loading control. (C) Summary for three independent experiments. Values are means ± SE, normalized against values of the vehicle control (0 μM). Asterisks indicate significance: P value of <0.05 by Student's t test against values of the vehicle control.