FIG 9.

CTT truncation (755*) does not increase infectivity or Env content of VLPs produced by transient transfection of 293T cells. Virions were pseudotyped with Comb-mut gp160 (full-length), CTT-truncated Comb-mut (L755stop), and a 10-fold excess of plasmid DNA of full-length gp160 and were compared to V4 hVLPs. Virions were normalized for particle concentration and then assayed for percent infectivity in TZM-bl reporter cells (A) as well as for Env incorporation (B). For the latter, virions were probed with anti-gp120 antibodies b12, 2G12, and 447-52D in an ELISA, and the optical density (OD) at 450 nm is shown. ns, not significant. ***, P < 0.001.