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Stability of synthetic transcripts. BSR cells were transfected in duplicates with each of the indicated mRNAs. Total cellular RNA was extracted at 4 and 8 hpt and used to amplify a fragment of the FLUC ORF, and as a control, a fragment of the GAPDH ORF, by RT-qPCR. For each transcript, the mRNA level at 8 hpt relative to that at 4 hpt was calculated using the 2−ΔΔCT equation. Data correspond to the averages and SD from at least 2 independent experiments.
