FIG 5.

Stability of synthetic transcripts. BSR cells were transfected in duplicates with each of the indicated RNAs. Total cellular RNA was extracted at 4 and 8 hpt and used to amplify a fragment of the FLUC and GAPDH ORFs by RT-qPCR (Materials and Methods). The mRNA level at 8 hpt relative to that at 4 hpt was calculated as for Fig. 2. Data correspond to the averages (±SD) from at least 2 independent experiments. Translation efficiency (T eff%) was estimated as the ratio between mean FLUC values (from Fig. 4) and the corresponding average of relative endpoint RNA levels (R). T eff for 5′wt/3′wt was set as 100%.