FIG 6.

Requirement of eIF4G for viral mRNA translation. (A) BSR cells were transfected with each of the indicated capped mRNAs, along with either a plasmid expressing poliovirus 2A protease (+) or pTM1 empty vector as a control (−), as indicated at the top. Cell lysates, harvested 8 h later, were subjected to Western blotting to detect eIF4G; actin was probed as a control for gel loading (upper blots). Cell lysates were also analyzed for FLUC activity, as described in legend to Fig. 1. Mean FLUC activity data (FLUC%, chart) from a representative experiment, carried out in triplicate, are presented as a percentage of the mean value determined for 5′wt/3′wt mRNA in the absence of 2APro, taken as 100%. (B) For each transcript, FLUC activity decrease (percent) was calculated by subtracting mean FLUC values in depleted cells from those determined in control cells (set as 100%). Data correspond to the means (±SD) from two independent experiments performed in triplicate.