FIG 4.

At early times p.i., all FXR and G3BP proteins colocalize with membrane-bound EEEV nsP3-containing pre-vRCs. (A) Schematic representation of the EEE/nsP3-Cherry/SINV genome. (B) NIH 3T3 cells were infected with EEE/nsP3-Cherry/SINV. At 3 h p.i., they were fixed and stained with antibodies specific to FXR1, FXR2 FMR1, G3BP1, G3BP2, and dsRNA. Nuclei were stained with Hoechst dye. Images are presented as multiple-image projections of 1-μm x-y sections at the plasma membrane. Pearson's colocalization coefficients are shown in the panels in the left column (mean ± SD, n > 7 cells). The four right columns present enlargements of the boxed regions in the panels in the left column. Bars, 10 μm (left column) and 3 μm (four right columns).