FIG 1.

Features of λ spanins. (A) The lysis cassette is shown. Lysis genes S (holin), R (endolysin), and Rz/Rz1 (the spanins) are under the control of the late promoter pR′. Rz1 is embedded in the +1 reading frame of Rz. The underlined nucleotides denote the ribosome binding site (RBS) and the start codon of Rz1. The translated products of Rz and Rz1 are shown above and below the nucleotide sequence, respectively. (B) A cartoon of spanin complex is shown within the cell envelope. The outer membrane (OM), inner membrane (IM), and predicted structural features of Rz and Rz1 are denoted on the left side of the cartoon. The relative positions of single missense mutations that inactivate Rz are represented by red and black rectangles on the right side of the cartoon. Red rectangles represent the relative positions of single missense mutations that were used in the suppressor screen. The relative positions of single missense mutations that inactivate Rz1 are represented by red and black circles on the right side of the cartoon. Red circles represent the relative positions of single missense mutations that were used in the suppressor screen. (C) A cartoon of Rz is shown with the percent probability of a coiled coil per residue, as predicted by MARCOIL, to the right of the cartoon.