TABLE 4.
Phages, strains, plasmids, and used in this study
| Name | Genotype and relevant features | Reference or source |
|---|---|---|
| Bacteriophages | ||
| λ900 | λΔ(stf tfa)::cat cI857 bor::kan, carries Camr and Kanr; wild-type RzRz1 | 7 |
| λ900 Rzam Rz1am | λΔ(stf tfa)::cat cI857 RzQ100am Rz1W38am bor::kan | 7 |
| λ cI857 | λ cI857 SRRzRz1 | Laboratory stock |
| λ910 | λ cI857 bor::kan; synthetic alleles of Rz and Rz1 (SynRz SynRz1)a | This study |
| Strains | ||
| MDS12 | MG1655 with 12 deletions, totalling 376,180 nucleotides, including all cryptic prophages | 16 |
| RY17341 | MDS12 ΔfhuA | 36 |
| MDS12 (λ900) | RY17341 lysogenized with λ900 | This study |
| MDS12 (λ900 Rzam Rz1am) | RY17341 lysogenized with λ900 Rzam Rz1am | This study |
| MDS12 (λ910) | RY17341 lysogenized with λ910 | This study |
| BW25113 | E. coli Keio collection parental strain | 37 |
| BW25113 (λ cI857) | BW25113 lysogenized with λ cI857 | This study |
| Plasmids | ||
| pER157 | pBR322 Δtet (SRRzRz1 bor::kan) | 7 |
| pAK1 | pER157 with tandem synthetic Rz and Rz1 genes | This study |
a
The Rz1 gene is located downstream of Rz (GenBank accession number KY609225).