TABLE 5.
Primers used in this study
| Primera | Sequence (5′–3′) |
|---|---|
| pRz S20P FOR | TCTGCCTGCCATGGGCTGTTAATC |
| pRz S20P REV | GATTAACAGCCCATGGCAGGCAGA |
| pRz V61A FOR | TCAGCGTGATGCTGCTGCGCTCGATGC |
| pRz V61A REV | GCATCGAGCGCAGCAGCATCACGCTGA |
| pRz L64H FOR | TGTTGCTGCGCACGATGCAAAAT |
| pRz L64H REV | ATTTTGCATCGTGCGCAGCAACA |
| pRz D65G FOR | GCTGCGCTCGGTGCAAAATACAC |
| pRz D65G REV | GTGTATTTTGCACCGAGCGCAGC |
| pRz K70E FOR | GCAAAATACACGGAGGAGTTAGCTG |
| pRz K70E REV | CAGCTAACTCCTCCGTGTATTTTGC |
| pRz L72F FOR | ACGAAGGAGTTCGCTGACGCC |
| pRz L72F REV | GGCGTCAGCGAACTCCTTCGT |
| pRz V86G FOR | ACGGGACGACGGGGCAGCCGGG |
| pRz V86G REV | CCCGGCTGCCCCGTCGTCCCGT |
| pRz L93S FOR | GCGGCGCCGATCACATATTAAGG |
| pRz L93S REV | CCTTAATATGTGATCGGCGCCGC |
| pRz Y127N FOR | AGCGAGACAACTTTACACTTAGGGAAAG |
| pRz Y127N REV | TCCCTAAGTGTAAAGTTGTCTCGCTCGGC |
| pRz G143R FOR | ACAACTGGAACGAACCCAGAAGTAT |
| pRz G143R REV | ATACTTCTGGGTTCGTTCCAGTTGT |
| pRz Y147H FOR | AACCCAGAAGCATATTAATGAGC |
| pRz Y147H REV | GCTCATTAATATGCTTCTGGGTT |
| pRz Q151R FOR | ATTAATGAGCGGTGCAGATAG |
| pRz Q151R REV | CTATCTGCACCGCTCATTAAT |
| pRz1 P32Q FOR | CGTGAAGCAACCACCGCC |
| pRz1 P32Q REV | GGCGGTGGTTGCTTCACG |
| pRz1 P33L FOR | GTGAAGCCACTGCCGCCTCCGGCG |
| pRz1 P33L REV | CGCCGGAGGCGGCAGTGGCTTCAC |
| pRz1 P35H FOR | CCACCACCGCATCCGGCGTGG |
| pRz1 P35H REV | CCACGCCGGATGCGGTGGTGG |
| pRz1 P36Q FOR | CCACCACCGCCTCAGGCGTGGATAATG |
| pRz1 P36Q REV | CATTATCCACGCCTGAGGCGCTGGTGG |
| pRz1 W46R FOR | CCGCCACCTGATCGGCAAACGCCAC |
| pRz1 W46R REV | GTGGCGTTTGCCGATCAGGTGGCGG |
| pRz1 I54N FOR | CTAAATGGAATCAATTCGCCATCGGAAAG |
| pRz1 I54N REV | CTTTCCGATGGCGAATTGATTCCATTTAG |
| pAK Rz1 E58K SDM | CCACTAAATGGAATCATATCGCCATCGAAAAGGGGATGAAAGCTTGCCC |
| Supp Seq REV | GAAGCGCGTGTGTATTGCTCAC |
| Lbd R S121 FWD | CAGCAATATCTGGGCTTCACTGC |
| AphI kan REV | GCAGTTTCATTTGATGCTCGATGAGTTTTTC |
| RzRz1 Seq FOR | CAAAATTCAAAGAAGCGGGCGGAAC |
| pAK Rz R37C FOR | AAGCCAGTGCGACAAAAATGCC |
| pAK Rz R37C REV | GCATTTTTGTCGCACTGGGCTTTG |
a
FOR, forward; REV, reverse.