FIG 9.

Schematic organization of a PEDV genome and the constructions of the full-length infectious cDNA clone. (A) Organization of a PEDV genome, including the ORF1a/b, spike (S), ORF3, envelope (E), membrane (M), and nucleocapsid (N) genes. (B) Five fragments comprising a full-length cDNA clone of PC22A. Two asterisks indicate BsaI sites in the PEDV genome that were removed by introducing silent mutations (nucleotide position 16507, C to G; nucleotide position 24332, A to G). In comparison with the genomic sequence of icPEDV (23), a GGC insertion is located at nucleotide position 22029, as indicated. (C) Mutant fragment E containing the 197-aa deletion in the S1 NTD. UTR, untranslated region; (A)23, 23-nt poly(A) tail.