TABLE 3.

General group information and clinical signs of Gn piglets after inoculation with recombinant viruses^a

Inoculum groupb	No. of piglets	Diarrhea rate (%)c,d	Mortality rate (%)d	Onset of diarrhea (dpi)	Highest FC score of each piglet	Duration of diarrhea (days)	Onset of fecal RNA shedding (dpi)f
icPC22A-S1Δ197	4	100 (4/4) A	0 (0/4) B	3.25 ± 0.50 A	2.50 ± 0.58	7.67 ± 0.58	3.25 ± 0.50
icPC22A	3	100 (3/3) A	100 (3/3) A	1.00 ± 0.00 B	3.00 ± 0.00	NA (>3)e	<1
Mock	2	0 (0/2) B	0 (0/2) B	ND	1.00	ND	ND

^aDifferent uppercase letters indicate a mean significant difference between groups (P < 0.05). ND, not detected.

^bPiglets in the icPC22A-S1Δ197 group were inoculated with 100 PFU/pig of virus; piglets in the icPC22A group were inoculated with 100 PFU/pig of virus; piglets in the mock inoculation group were inoculated with PBS.

^cFecal consistency (FC) was scored as follows: 0, solid, 1, pasty, 2, semiliquid; and 3, liquid. An FC score of ≥2 was considered diarrhea.

^dValues in parentheses are the number of positive results/number of animals tested.

^eNA, not available. Since all icPC22A-inoculated piglets became moribund and were euthanized by 2dpi, this value could not be determined.

^fThe detection limit of PEDV real-time RT-PCR corresponds to 4.8 log₁₀ GE/ml.