FIG 6.

) CXCR5⁺ CD4⁺ T cells derived from HIV controllers provide B cell help superior to that from cells derived from HIV progressors. Naive B cells isolated from peripheral blood were cocultured with autologous CXCR5-enriched CD4 T cells for 7 days in the presence of CD28/CD49d costimulating antibodies and SEB or HIV-1 PTE ENV peptide pools. (A) Naive B cells cocultured with CXCR5⁺ CD4⁺ T cells in the presence of SEB lose expression of IgD and increase expression of IgG similarly in HIV progressors and HIV controllers. (B) No difference in phenotypic changes of B cells in expression of CD38, CD138, or PDL1 expression after stimulation with SEB between HIV controllers and HIV progressors. (C) Significant differences between HIV controllers and HIV progressors in the class switching of naive B cells cocultured with Env-stimulated CXCR5⁺ CD4⁺ T cells. (D) No differences in phenotypic changes of B cells in CD38, CD138, or PDL1 expression after stimulation with ENV PTE peptide pools between HIV controllers and HIV progressors. Data are presented as boxes and whiskers and groups compared by Mann-Whitney tests.