Table 1.
Details of the primers used for qRT-PCR normalization.
| Gene abbreviations | Gene name | Accession No. | Length (bp) | Primer sequence U/L [5′–3′] | Arabidopsis ortholog No | Identity (%) | Tm°C | E (%) | R2 |
|---|---|---|---|---|---|---|---|---|---|
| eIF2 | Eukaryotic transation initiation factor | c40298.graph_c0 | 117 | For:TACCAGTGGCTCGCTTGAC | At5g20920.3 | 87 | 83 | 1.20 | 0.9583 |
| Rev:CAACCAAAGCAAATGACGTACTC | |||||||||
| ACT7 | Actin7 | AY870652.1 | 181 | For: AGGAATCGCTGACCGTATG | At5g09810.1 | 93 | 85.5 | 1.15 | 0.9829 |
| Rev : TGGACCCGACTCATCGTATT | |||||||||
| PP2A-4 | Protein phosphatase 2A-4 | c41201.graph_c0 | 151 | For: GAATGCCTGCGAAAGTATGG | At3g58500.1 | 93 | 83 | 1.12 | 0.9859 |
| Rev: TCCTAATGTTGTCAAGGGTCTC | |||||||||
| AP-2 | AP-2 complex | c47959.graph_c0 | 137 | For: AAGACCTTCAGCCTTACGCC | At5g22780.1 | 92 | 83 | 1.35 | 0.9849 |
| Rev: ACTGCATCGAGGTTGTCTGG | |||||||||
| APT3 | Adenine phosphoribosyl transferase | c37104.graph_c0 | 178 | For:CCGTGTCGTTCCAGATTTTC | At4g22570.1 | 93 | 82 | 1.04 | 0.9983 |
| Rev:GATTGGTGGACCGAATAGGA | |||||||||
| UBC29 | Ubiquitin-conjugating enzyme 29 | c38774.graph_c0 | 148 | For:TTCTTAAAGACCAGTGGAGC | At2g16740.1 | 90 | 83.5 | 0.97 | 0.9996 |
| Rev:ATGGCTTCGTATTTGACCTT | |||||||||
| RPL15 | Ribosomal protein L | c42633.graph_c1 | 133 | For:GGGCGAAGAGGAAAGGTAG | At3g25920.1 | 87 | 84.6 | 1.05 | 0.9920 |
| Rev: CGGAAGACGGCGATAAAGAG | |||||||||
| UBC22 | Ubiquitin-conjugating enzyme 22 | c38263.graph_c0 | 225 | For:GTATGAAGTTGGCATTGTCGC | At5g05080.2 | 89 | 83 | 0.89 | 0.9990 |
| Rev:TCTTTCCAGCCTGTTCGTTT | |||||||||
| UBC19 | Ubiquitin-conjugating enzyme 19 | c39354.graph_c0 | 188 | For: TGAGACATGCTGCTTCCATC | At3g20060.1 | 92 | 84 | 0.94 | 0.9845 |
| Rev: CTTCTTGGTTGCTCCAAAGC | |||||||||
| TUB4 | Tubulin | c43494.graph_c0 | 85 | For:GCCCCGATAACTTCGTCTT | At5g44340.1 | 93 | 85.8 | 1.12 | 0.9790 |
| Rev: TCAATCAACTCCGCTCCCT | |||||||||