Table 4.
Ranking of the candidate reference genes and their expression stability values calculated using BestKeeper.
| Gene | UBC29 | AP-2 | TUB4 | ACT7 | UBC19 | APT3 | eIF2 | PP2A-4 | RPL15 | UBC22 | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Total | SD[±CP] | 1.46 | 0.84 | 1.10 | 1.07 | 1.10 | 2.59 | 1.14 | 0.97 | 1.08 | 1.94 |
| CV | 5.96 | 3.49 | 4.80 | 5.18 | 4.47 | 10.41 | 5.01 | 4.47 | 4.82 | 8.32 | |
| Tissue | SD[±CP] | 0.47 | 0.72 | 0.76 | 0.81 | 0.86 | 0.86 | 0.99 | 0.99 | 1.09 | 1.63 |
| CV | 2.02 | 3.06 | 3.37 | 3.99 | 3.59 | 3.77 | 4.47 | 4.57 | 4.85 | 7.11 | |
| Abiotic stress | SD[±CP] | 1.20 | 0.75 | 1.38 | 1.10 | 1.31 | 1.76 | 1.16 | 0.94 | 1.01 | 2.54 |
| CV | 4.54 | 3.08 | 5.86 | 5.14 | 5.15 | 6.22 | 4.86 | 4.30 | 4.51 | 10.62 | |
The value of SD[±CP] below 1.0, marked in bold, indicate that the reference gene is qualified, and the value of CV is used to rank the reference genes.