FIG 4.

Capillary electrograms and graphical presentations of RNA metabolism activities of Rv2783. (A and B) Representative capillary electrograms showing the uninhibited RNA polymerization activity of wild-type Rv2783 (A) and inhibition of RNA polymerization activity of wild-type Rv2783 by POA (B). (C) Comparison of the effects of POA and PZA on the RNA polymerization activity of the wild-type Rv2783 protein. (D and E) Representative capillary electrograms showing the uninhibited RNA polymerization activity of mutant Rv2783_Asp67Asn (D) and inhibition of the RNA polymerization activity of mutant Rv2783_Asp67Asn by POA (E). (F) Comparison of the effects of POA and PZA on the RNA polymerization activity of the mutant Rv2783_Asp67Asn protein. (G and H) Representative capillary electrograms showing the uninhibited RNA phosphorolysis activity of wild-type Rv2783 (G) and inhibition of the RNA phosphorolysis activity of wild-type Rv2783 by POA (H). (I) Comparison of the effects of POA and PZA on the RNA phosphorolysis activity of the wild-type Rv2783 protein. For panels C, F, and I, the data are presented as means ± SEMs. Student's t test was used for the statistical analysis.