FIG 7.

Comparative study of ROS, mitochondrial peroxidase activity, and GSH content after incubation with AmB and enzyme-specific inhibitors in both the AmB-sensitive and -resistant strains. (A) Fluorometric assay (using the H₂DCFDA fluorogenic probe) to assess the content of ROS in AmB-sensitive and -resistant isolates after incubation of AmB and inhibitors. (B) Assessment of peroxidase activities of AmB- and inhibitor-preincubated AmB-sensitive and -resistant isolates using the fluorogenic peroxidase substrate dihydrorhodamine 123 (DHR). (C) The effect of inhibitors on cellular GSH content in AmB-sensitive and -resistant isolates was observed. MCB, monochlorobimane dye. Asterisks indicate significant difference: *, P < 0.05; **, P < 0.001.