TABLE 6.
Activity of elbasvir in GT4 NS5A RASsa
| Replicon | EC (nM) ± SD and fold shift |
|||
|---|---|---|---|---|
| EC50 | EC50 fold shift | EC90 | EC90 fold shift | |
| Wild type (ED43) | 0.0002 ± 0.0002 | 1 | 0.0008 ± 0.0001 | 1 |
| L30F | 0.003 ± 0.002 | 15 | 0.015 ± 0.011 | 18.8 |
| L30P | 0.0002 ± 0.0001 | 1 | 0.0004 ± 0.0002 | 0.5 |
| L30S | 0.0008 ± 0.0005 | 4 | 0.0043 ± 0.0030 | 5.4 |
| M31V | 0.0005 ± 0.0004 | 2.5 | 0.0009 ± 0.0007 | 1.1 |
| N69K | 0.0003 ± 0.0002 | 1.5 | 0.0003 ± 0.0001 | 0.4 |
| Y93H | 0.0015 ± 0.0002 | 7.5 | 0.0032 ± 0.0030 | 4 |
a
The chimeric replicons bearing the resistance-associated substitutions were generated in a GT2a (JFH1) background, and potencies were determined using a TaqMan-based assay. The fold shift is based on the wild-type value.