TABLE 3.
Susceptibilities to terbinafine and itraconazole of A. vanbreuseghemii transformants expressing mutated forms of the SQLE gene and corresponding expression levels
| Species and strain | Amino acid substitution | MIC (μg/ml) |
Fold expression of SQLE (mean ± SD)a | |
|---|---|---|---|---|
| Terbinafine | Itraconazole | |||
| T. interdigitale | ||||
| ATCC MYA-4439 | None | 0.00625 | 0.12 | ND |
| A. vanbreuseghemii | ||||
| 1062Av1401 | None | 0.0125 | 0.06 | 1.02 ± 0.24 |
| Av-FRT-1-3 | None | 0.00625 | 0.12 | 1.28 ± 0.06 |
| Av-S38A | Leu393Phe | 1.6 | 0.12 | 1.08 ± 0.11 |
| Av-1-3 | Leu393Ser | 0.4 | 0.12 | ND |
| Av-S714J6 | Phe397Leu | 3.2 | 0.12 | 1.37 ± 0.11 |
| Av-7-5 | Phe397Ile | 1.6 | 0.06 | ND |
| Av-S28 M | Phe415Val | 0.4 | 0.12 | ND |
| Av-2-4 | His440Tyr | 0.2 | 0.12 | ND |
| T. rubrum | ||||
| Tr-FRT-52-9 | None | 0.00625 | 0.06 | 1.27 ± 0.14 |
| Tr-T31C | Leu393Phe | 3.2 | 0.12 | 1.63 ± 0.18 |
| Tr-2-3 | Leu393Ser | 0.4 | 0.06 | ND |
| Tr-T719J | Phe397Leu | 3.2 | 0.06 | 1.35 ± 0.16 |
| Tr-4-1 | Phe397Ile | 1.6 | 0.06 | ND |
| Tr-T4B | Phe415Val | 0.4 | 0.12 | ND |
| Tr-2-1 | His440Tyr | 0.05 | 0.25 | ND |
a
Results are from three independent real-time PCR experiments. ND, not determined. Expression levels of SQLE genes are indicated as relative fold changes compared to the CT mean of the parent strain 1062Av1401 data.