TABLE 1.
Characteristica | ICBEC2AM | ICBEC3AM | ICBEC13AM | ICBEC7P | ICBEC72H |
---|---|---|---|---|---|
Source | Seawater | Seawater | Seawater | Infected migratory penguin | Human infection |
Location | −23.974697S, −46.395060W | −23.974995S, −46.371613W | −23.986450S, −46.309086W | −23.986306S, −46.308361W | −5.779257S, −35.200916W |
Isolation date | September 2016 | September 2016 | September 2016 | June 2013 | March 2016 |
NaCl tolerance (%) | 10 | 10 | 10 | 10 | 10 |
Serotype | ONT:H55 | O9:H4 | O54:H32 | ONT:H32 | ONT:H9 |
ST/CC | 1638 | 46/46 | 10/10 | 10/10 | 101/43 |
Virulence genes | Not detected | iss, gad, mchF | gad | gad | iroN, mcmA, mchB, mchC, mchF, lpfA, iss |
Phylogroup | B1 | B1 | B1 | A | B1 |
Resistance | AMO, AMP, CAZ, CEF, CRO, CTF, CTX, DOX, NAL, SUL, TET | AMO, CEF, CLO, NAL, SUL, SXT | AMO, AMP, ATM, CAZ, CEF, CRO, CTX, DOX, NAL, SUL, SXT, TET | AMK, AMO, AMP, ATM, CAZ, CEF, CIP, CTF, CTX, ENR, FEP, GEN, NAL, SXT, TET | AMO, AMP, ATM, CEF, CTX, FEP |
Colistin/polymyxin MIC (μg/ml) | 4/4 | 4/4 | 4/4 | 8/8 | 4/4 |
Resistance genotype | mcr-1, blaCTX-M-8, qnrB19, aadA2, strA, strB, sul2 | mcr-1, blaTEM-1B, qnrB19, catA1, aadA1, strA, strB, sul1, sul2, tetA, dfrA1, dfrA8 | mcr-1, blaCTX-M-1, aadA1, sul2, tetA, tetB | mcr-1, blaCTX-M-1, aadA1, sul2, tetA, tetB | mcr-1, blaCTX-M-8 |
Plasmids (Inc)b | I1, ColRNAI, X4 | FIB, Q1, X4 | HI2, I1, N, X4 | FIN, HI2, HI2A, I1, N, X4 | I1, X4 |
E. coli isolates ICBEC2AM, ICBEC3AM, and ICBEC13AM were analyzed in this study. Data for E. coli ICBEC7P and ICBEC72H were obtained from earlier studies by our group (5, 7). ST, sequence type; CC, clonal complex; AMK, amikacin; AMO, amoxicillin; AMP, ampicillin; ATM, aztreonam; CAZ, ceftazidime; CEF, cephalothin; CIP, ciprofloxacin; CLO, chloramphenicol; CRO, ceftriaxone; CTF, ceftiofur; CTX, cefotaxime; DOX, doxycycline; ENR, enrofloxacin; FEP, cefepime; GEN, gentamicin; NAL, nalidixic acid; SUL, sulfonamide; SXT, trimethoprim-sulfamethoxazole; TET, tetracycline.
The replicon types of plasmids carrying the mcr-1 gene are in bold.