FIG 2.

Competitive fitness of isogenic strains containing mutated MRR1 alleles. An RFP-labeled derivative of strain SC5314 (SCADH1R1A, light gray bars) was mixed with two independently generated strains in which the endogenous MRR1 alleles in strain SC5314 were replaced by the indicated mutant alleles or a wild-type copy without a mutation (dark gray bars). Shown are the relative proportions of the strains in the inoculum (day 0) and after two rounds of coculture (day 1 and day 2) at 30°C in YPD medium. Results are the means and standard deviations from four coculture experiments (two with strain A and two with strain B). The control strains were each tested six times; i.e., results are from 12 cocultures. Significant differences in the proportions of the test strains at day 1 and day 2 compared to day 0 are indicated by asterisks (P < 0.05). See Data Set S2 for details.