FIG 3.

Competitive fitness of fluconazole-susceptible clinical isolates F2 and 5833 (light gray bars) and genetically engineered derivatives carrying the MRR1 GOF mutations from matched resistant isolates F5 and 6692, respectively (dark gray bars). Two independently generated RFP-labeled derivatives of isolates F2 and 5833 were each mixed with two independently constructed corresponding mutants; i.e., four coculture experiments were performed for each comparison. Shown are the relative proportions of the strains in the inoculum (day 0) and after two rounds of coculture (day 1 and day 2) at 30°C in YPD medium. Results are the means and standard deviations from the four coculture experiments. Significant differences in the proportions of the strains with MRR1 GOF mutations at day 1 and day 2 compared to day 0 are indicated by asterisks (P < 0.05). See Data Set S3 for details.