Fig. 2.

Ovarian expression of PPARγ, COX-2 and StAR proteins during hCG-triggered ovulation and luteinization. hCG-treated ovaries were sampled at the indicated time points for immunohistochemical (only for PPARγ) and Western blot analyses of PPARγ, COX-2 and StAR. Immunoreactive PPARγ was abundant in granulosa cells of preovulatory follicles at hCG0h (A, B), but markedly reduced in granulosa cells at hCG8h (C) and granulosa-lutein cells at hCG24h (D). Scale bars: 50 µm (A, C, D), 10 µm (B). Expression levels of three proteins were normalized to the internal standard (β-actin) (E). Data are mean with SEM (n=3 per time point). *, P<0.05 versus each value at hCG0h.