Table 1. Antibodies used in immunohistochemistry.
| Antigen | Host species | Clonality | Clone name | Dilution | Antigen retrieval | Manufacture |
|---|---|---|---|---|---|---|
| Cytokeratin (AE1/AE3) | mouse | monoclonal | AE1/AE3 | 1:50 | Microwaving pH6b) | Dako (Glostrup, Denmark) |
| Cytokeratin CAM5.2 | mouse | monoclonal | CAM5.2 | undiluted | Microwaving pH6b) | BD Biosciences (San Jose, CA, U.S.A.) |
| Cytokeratin 7 (CK7) | mouse | monoclonal | OV-TL 12/30 | 1:100 | Proteinase Kd) | Dako (Glostrup, Denmark) |
| Human chorionic gonadotropin (hCG) | rabbit | polyclonal | n.a. | 1:1,000 | Autoclaving pH9a) | Dako (Glostrup, Denmark) |
| α-1-fetoprotein (AFP) | rabbit | polyclonal | n.a. | 1:400 | Microwaving pH6b) | Dako (Glostrup, Denmark) |
| Octamer-binding transcription factor-3/4 (OCT3/4) | mouse | polyclonal | n.a. | 1:100 | None | Santa Cruz Biotechnology, Inc. (Dallas, TX, U.S.A.) |
| Human placental alkaline phosphatase (hPLAP) | mouse | monoclonal | 8A9 | 1:25 | Autoclaving pH9a) | Dako (Glostrup, Denmark) |
| α-inhibin | rabbit | polyclonal | n.a. | 1:100 | Autoclaving pH9a) | Gift from Dr. Gen Watanabe. |
| Vimentin | goat | polyclonal I | n.a. | 1:200 | Microwaving pH6b) | Santa Cruz Biotechnology, Inc. (Dallas, TX, U.S.A.) |
| Ionized calcium-binding adaptor molecule 1 (Iba-1) | rabbit | polyclonal I | n.a. | 1:300 | None | Wako Pure Chemical Industries Ltd. (Osaka, Japan) |
| Proliferating cell nuclear antigen (PCNA) | mouse | monoclonal | PC10 | 1:200 | None | Dako (Glostrup, Denmark) |
| Ki-67 | mouse | monoclonal | MIB1 | 1:200 | Autoclaving pH6c) | Dako (Glostrup, Denmark) |
Abbreviation: n.a, not applicable. a) Autoclaved at 121°C for 10 min in Dako target retrieval solution (pH 9.0). b) Microwaved at 90°C for 10 min in 10 mM citrate buffer (pH 6.0). c) Autoclaved at 121°C for 10 min in 10 mM citrate buffer (pH 6.0). d) Incubated with proteinase K solution (20 µg/ml) for 15 min.