Fig. 1.

The TLC shows the glycolipid standards glucose–octanoate (1) and xylose–octanoate (2), synthesized with vinyl-octanoate. S is a lab-intern rhamnolipid standard, which acts as a positive control for the TLC. P1–P4 shows extracts of the reaction in a CC:sugar mix DES with vinyl-octanoate as substrate. At a Rf between 0.1 and 0.2 there is mainly the sugars, glucose and xylose, out of the DES, which are comparable with the pure sugar standards of glucose (Gluc) and xylose (Xyl). The brownish double spots and the purple spots (P1–P4) represent different glucose–octanoates (Rf 0.4–0.5) and the slightly spot with a Rf of 0.65–0.75 xylose–octanoate. The spot on the height of the running front indicates more non-polar products, like di- or poly-acylated sugars. A negative control without enzyme and without lipase shows only glucose and xylose spots (Contr.)