Fig. 3.

Effect of steroids on culture-induced changes in ESR1, PTGS2 and OXTR mRNAs in vitro. Relative mRNA abundance of ESR1, PTGS2 and OXTR was measured in term non-laboring myometrial tissue samples following 48 h incubation in the presence of 500 nM progesterone (P4) or 500 nM P4 + 400 nM estradiol (E2) (n=3), and expressed relative to Alien reference. (A) ESR1 mRNA abundance. (B) PTGS2 mRNA abundance. (C) OXTR mRNA abundance. Data were logarithmically transformed to approach normal distribution (Shapiro-Wilk normality test) then compared by 1-way ANOVA with multiple comparisons (Dunnett). Data are mean ± S.E.M.