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. 2017 Jun 23;53:73–89. doi: 10.1540/jsmr.53.73

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©2017 The Japan Society of Smooth Muscle Research

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 5. — Effect of cAMP and PMA on culture-induced changes in ESR1, PTGS2 and OXTR mRNA in vitro. Relative mRNA abundance of ESR1, PTGS2 and OXTR was measured in term non-laboring myometrial tissue samples (n=3) following 48 h incubation in the presence of 8-Br-cAMP (250 µM) or PMA (0.1 and 1.0 µM), and expressed relative to Alien reference. (A) Effect of 8-Br-cAMP on ESR1 mRNA abundance. (B) Effect of PMA on ESR1 mRNA abundance. (C) Effect of 8-Br-cAMP on PTGS2 mRNA abundance. (D) Effect of PMA on PTGS2 mRNA abundance. (E) Effect of 8-Br-cAMP on OXTR mRNA abundance. (F) Effect of PMA on OXTR mRNA abundance. Data was checked for normality (Shapiro-Wilk normality test) and if necessary was logarithmically transformed to approach normal distribution (Shapiro-Wilk normality test). 8-Br-cAMP data was analysed using paired t-test, whilst 1-way ANOVA with multiple comparisons (Dunnett) was used to analyse PMA data. Data are mean ± S.E.M.