Table 1.
Year | n | Blood sampling | Long-term storage | Extraction | |||
---|---|---|---|---|---|---|---|
State | Conditions | Duration | Timing | Method | |||
(a) | |||||||
1998 | 13 | Needle and syringe | Extracted DNA in Tris_EDTA low buffer | Frozen at −20 and −80 °C | 14–15 years | Prior to long-term storage | Modified Chelex protocol |
1999 | 2 | ||||||
2000 | 56 | Needle and syringe | Red blood cells in Tris–EDTA-SDS buffer | Frozen at −2 and −80 °C | 13 years | Prior to qPCR | Nucleospin® Blood Kit |
2012 | 108 | Pinprick with needle | Whole blood on FTA®ClassicCards | Separate ziplock bags at room temp | 1 year | Prior to qPCR | Nucleospin®
Tissue Kit |
Year | n | Blood sampling | Long-term storage | Extraction | |||
---|---|---|---|---|---|---|---|
State | Conditions | Duration | Timing | Method | |||
(b) | |||||||
2014 | 30 | Needle and capillary | Extracted DNA | Frozen at −80 °C | 2 months | Prior to long-term storage | Nucleospin® Blood QuickPure Kit |
2014 | 30 | Needle and capillary | Whole blood | Frozen at −80 °C | 2 months | Prior to qPCR | Nucleospin® Blood QuickPure Kit |
2014 | 30 | Needle and capillary | Whole blood on FTA®ClassicCards | Storage at room temp | 2 months | Prior to qPCR | Nucleospin® Blood QuickPure Kit |