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. 2017 May 25;184(2):341–350. doi: 10.1007/s00442-017-3887-3

Table 1.

Different sample collection, processing, storage, and DNA extraction methods used for A wandering albatross blood samples collected at Bird Island, South Georgia, B zebra finch blood samples, which were used to measure relative telomere length

Year n Blood sampling Long-term storage Extraction
State Conditions Duration Timing Method
(a)
1998 13 Needle and syringe Extracted DNA in Tris_EDTA low buffer Frozen at −20 and −80 °C 14–15 years Prior to long-term storage Modified Chelex protocol
1999 2
2000 56 Needle and syringe Red blood cells in Tris–EDTA-SDS buffer Frozen at −2 and −80 °C 13 years Prior to qPCR Nucleospin® Blood Kit
2012 108 Pinprick with needle Whole blood on FTA®ClassicCards Separate ziplock bags at room temp 1 year Prior to qPCR Nucleospin®
Tissue Kit
Year n Blood sampling Long-term storage Extraction
State Conditions Duration Timing Method
(b)
2014 30 Needle and capillary Extracted DNA Frozen at −80 °C 2 months Prior to long-term storage Nucleospin® Blood QuickPure Kit
2014 30 Needle and capillary Whole blood Frozen at −80 °C 2 months Prior to qPCR Nucleospin® Blood QuickPure Kit
2014 30 Needle and capillary Whole blood on FTA®ClassicCards Storage at room temp 2 months Prior to qPCR Nucleospin® Blood QuickPure Kit