Fig. 2.

Lung gene expression analysis in murine OVA asthma models. a, left Hierarchical clustering of 361 probe sets showing at least twofold regulation (PBS treated vs OVA treated) with P ≤ 0.05 (the Student t test). a, right Highlight of cluster containing the most highly induced genes by OVA treatment, yet showing preferential normalization in response to 51D9. The positions of CLCA3 (the murine equivalent to human CLCA1) and Muc5ac, which were taken forward into human studies, are indicated. b Highly inducible mRNAs are particularly sensitive to dual receptor blockade. To highlight the effect of dual receptor blockade, the antibody response profile for each of the 20 mRNAs most highly induced in OVA-treated lungs compared with control lungs is indicated. Induced genes were defined as those upregulated at least threefold with P ≤ 0.01 using the Student t test. Values were normalized by subtraction of the PBS control. CLCA calcium-activated chloride channel regulator, IL interleukin, mRNA messenger RNA, Muc5ac mucin 5ac, OVA ovalbumin, PBS phosphate-buffered saline, R receptor