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. 2017 Feb 20;74(13):2513–2524. doi: 10.1007/s00018-017-2472-6

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — Confocal analysis of ABCB4 variant sub-cellular localisation. Trafficking of ABCB4 mutants D243A, K435T, G535D, R545C, I490T, and S978P was examined by confocal microscopy (shown in magenta in the merged images) in cells co-expressing ATP8B1/CDC50. The Na⁺/K⁺ ATPase, shown in green in the merged images, marks the plasma membrane. Co-localisation in the merged image is white when the pixels for the Na⁺/K⁺ ATPase and ABCB4 overlap and have the same intensity. The Mander’s coefficient for the given field of view provides a measure of this co-localisation above a calculated threshold, irrespective of pixel intensity