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. 2017 Feb 8;21(7):1411–1419. doi: 10.1111/jcmm.13070

Figure 2.

Figure 2

APN promoted h‐JBMMSCs up‐regulating CXCL1 and CXCL8. (A) h‐JBMMSCs were cultured in osteoblast‐inducing conditional media with or without APN (1 μg/ml) for 1 week, and the total RNA was analysed using RNA‐seq. A total of 198 genes showing differential expression were identified, including 185 up‐regulated and 13 down‐regulated genes in the APN‐treatment group (P < 0.005). The CXCL1 and CXCL8 were selected as the target genes. To validate the results of RNA‐seq, real‐time PCR (B) and (C) ELISA were used to measure the expression of CXCL1 and CXCL8 in the control and APN‐treated groups (n = 3; *P < 0.05; **P < 0.01). (D) The exogenous CXCL1 or CXCL8 proteins were added in the lower compartment to verify its chemotaxis effect. The cell number was displayed as mean ± standard deviation (n = 3; *P < 0.05). (E) The Western blot was used to evaluate the expression of CXCR1 and CXCR2, the specific receptor of CXCL1 and CXCL8, after different treatments (n = 3; *P < 0.05; **P < 0.01).