Figure 3.

Adoptive transfer of NK1.1⁻ CD4⁺ NKG2D⁺ cells suppressed DSS‐induced colitis. (A) Weight curve of DSS‐mice treated with NK1.1⁻ CD4⁺ NKG2D⁺ cells, NK1.1⁺ CD4⁺ NKG2D⁺ cells or NK1.1⁻ CD4⁺ NKG2D⁺ cells pre‐incubated with TGF‐β antibody. (B) Disease activity index of all groups of mice with colitis. (C) Morphological changes of colons with a variety of stimulations. * represents comparison between the DSS‐treated group and the NK1.1⁻ CD4⁺ NKG2D⁺ cell transferred DSS‐treated group. (D) Histology of colon sections in various treatments (×200). (E) CD4⁺ NKG2D⁺ T cells sorted by magnetic‐labelled antibody were injected into the tail veins of DSS‐treated mice on days 1, 3 and 5. On day 7, the mice were killed to obtain mononuclear cells. The frequency of 7‐AAD ⁻ CD45⁺ NK1.1⁻ CD4⁺ NKG2D⁺ cells was analysed. (F) TGF‐β staining of colonic NK1.1⁻ CD4⁺ NKG2D⁺ cells. The experiment was repeated thrice.