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. 2016 Nov 10;21(7):1266–1279. doi: 10.1111/jcmm.12999

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© 2016 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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hnRNPK knockdown induces G0/G1 arrest in bladder cancer cells. (A and B) Flow cytometry analysis of UM‐UC‐3 and T24 cells transfected with si‐hnRNPK or control siRNA for 48 hrs. The percentages (%) of cell populations at different stages of the cell cycle are listed in the panels. All histograms show the percentage (%) of cell populations from three independent experiments. (C and D) EdU assay measurement of the cell population in the S phase. Blue, nucleus; red, S‐phase cells (EdU‐positive). Histological analysis of the percentage of EdU‐positive cells in control and hnRNPK knockdown cells is shown. The results are presented as the means ± S.D. of three independent experiments. *P < 0.05, **P < 0.01.