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. 2017 Feb 3;21(7):1315–1328. doi: 10.1111/jcmm.13061

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© 2017 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Nephrin increased from post‐natal Day 1 to adulthood during renal development. (A) Nephrin expression was assessed by Western blot assay on post‐natal day 1(P1), P5, P7, P14, P28 and P49 in renal cortex during kidney development. (B) Quantitative determination of Western blot assays for nephrin as beta‐actin was a consult. (C) Immunofluorescence staining of nephrin (Alexa 594, red) in different development stages. Scale bar: 30 μm. (D) Fluorescent intensity of nephrin in glomeruli. Data were presented as the means ± S.D. n = 6/group. *P < 0.05.