Circular phylogenetic tree including all detected and reference sequences. The evolutionary history was inferred using the Neighbor-Joining method (Saitou and Nei, 1987). The analysis involved 302 nucleotide sequences downloaded from the GenBank database including two representative sequences from each Mycoplasma or Ureaplasma species and all unclassified species. Our sequences clustered into three significant clusters depicting sequences clustering with Mycoplasma hominis strains (Subtree 1), with Candidatus Mycoplasma girerdii strains, and strains of novel Mycoplasma species (Subtree 2) and with Ureaplasma spp. strains (Subtree 3). Accession numbers of the reference sequences used are the following: AB299548.1, AB299549.1, AB558897.1, AB558898.1, AB558899.1, AB576869.1, AB617737.1, AB617738.1, AB680604.1, AB680624.1, AB680625.1, AB680668.1, AB680669.1, AB680678.1, AB680679.1, AB680680.1, AB680681.1, AB680682.1, AB680683.1, AB680684.1, AB680685.1, AB680687.1, AB680688.1, AB680689.1, AB680690.1, AB680691.1, AB680692.1, AB680693.1, AB680694.1, AB725596.1, AB740010.1, AB740012.1, AB758439.1, AB758440.1, AB848713.1, AF001173.1, AF009831.1, AF009832.1, AF042194.1, AF060821.1, AF064062.1, AF125584.1, AF125585.1, AF125587.1, AF125588.1, AF125589.1, AF125592.1, AF125593.1, AF125878.1, AF125879.1, AF132740.1, AF132741.1, AF178676.1, AF212859.1, AF221111.1, AF221112.1, AF221113.1, AF221114.1, AF221115.1, AF221116.1, AF221117.1, AF221118.1, AF221119.1, AF221120.1, AF221121.1, AF261729.1, AF261730.1, AF304323.1, AF304324.1, AF304325.1, AF306346.1, AF538681.1, AF538682.1, AF538683.1, AF538684.1, AF538961.1, AJ002265.1, AJ419900.1, AJ419903.1, AJ419905.1, AM073012.2, AM073013.1, AM073015.1, AM745338.1, AM774638.1, AY050170.1, AY121107.1, AY121108.1, AY150066.1, AY171918.1, AY191226.1, AY366210.1, AY383241.1, AY466443.1, AY529641.1, AY531655.1, AY714305.2, AY756171.1, DQ464424.1, DQ464425.1, DQ641256.1, DQ653410.1, DQ840512.1, DQ840513.1, E02783.1, EF036469.1, EF577506.1, EU646197.1, EU646198.1, EU789558.1, EU789559.1, EU888930.1, FN392885.1, FN392886.1, FN436019.1, FN908083.1, FN908084.1, FN984917.1, GU124613.1, GU124614.1, GU227372.1, GU227388.1, GU227389.1, GU227390.1, GU227391.1, GU227392.1, GU227393.1, GU227394.1, GU227395.1, GU227396.1, GU227397.1, GU227398.1, GU230144.1, GU562823.1, GU569852.1, GU569853.1, GU905011.1, GU905012.1, HM235423.1, HQ634379.1, HQ634380.1, JN214358.1, JN214359.1, JN644767.1, JN644768.1, JN935881.1, JN935892.1, JN935893.1, JQ689949.1, JQ689950.1, JQ897386.1, JQ897387.1, JQ897388.1, KC512403.1, KC512404.1, KF419350.1, L08054.1, L22210.1, L24103.1, L33760.1, L33765.1, M23939.2, M86340.1, NR_024977.1, NR_024978.1, NR_024979.1, NR_024981.1, NR_024982.1, NR_024983.1, NR_024984.1, NR_024985.1, NR_024986.1, NR_024987.1, NR_024988.1, NR_025055.1, NR_025061.1, NR_025062.1, NR_025063.1, NR_025064.1, NR_025065.1, NR_025068.1, NR_025069.1, NR_025070.1, NR_025071.1, NR_025133.1, NR_025134.1, NR_025135.1, NR_025176.1, NR_025177.1, NR_025178.1, NR_025179.1, NR_025180.1, NR_025181.1, NR_025182.1, NR_025183.1, NR_025184.1, NR_025185.1, NR_025186.1, NR_025187.1, NR_025188.1, NR_025896.1, NR_025912.1, NR_025913.1, NR_025914.1, NR_025954.1, NR_025963.1, NR_025964.1, NR_025965.1, NR_025966.1, NR_025968.1, NR_025971.1, NR_025984.1, NR_025985.1, NR_025986.1, NR_025987.1, NR_025988.1, NR_025989.1, NR_026017.1, NR_026034.1, NR_026035.1, NR_026036.1, NR_026037.1, NR_026155.1, NR_029174.1, NR_029175.1, NR_029180.1, NR_029181.1, NR_029183.1, NR_036954.1, NR_037123.1, NR_041844.1, NR_041846.1, NR_042948.1, NR_043138.1, NR_044664.1, NR_044668.1, NR_044673.1, NR_044767.1, NR_044772.1, NR_044811.1, NR_074135.1, NR_074289.1, NR_074301.1, NR_074611.1, NR_074620.1, NR_102477.1, NR_103942.1, NR_104953.1, U02968.1, U04644.1, U04645.1, U04646.1, U04648.1, U04649.1, U04650.1, U04651.1, U04652.1, U04653.1, U04654.1, U04655.1, U04656.1, U09786.1, U09787.1, U09788.1, U15794.1, U15795.1, U16323.1, U16758.1, U16759.1, U16760.1, U19768.1, U22013.1, U22415.1, U26036.1, U26042.1, U26043.1, U26045.1, U26047.1, U26049.1, U26051.1, U26053.1, U26055.1, U29676.1, U44763.1, U44764.1, U44765.1, U44768.1, U44769.1, U44771.1, U56733.1, U58504.1, U58997.1, U67943.1, U67944.1, U67945.1, U67946.1, U83502.1, U83663.1, X00921.1, X62699.1, X76560.1, and Y00149.1.